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KMID : 0617819940040010043
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Journal of Wonkwang Oriental Medicine
1994 Volume.4 No. 1 p.43 ~ p.51
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ALLERGEN CHALLENGE IN OVALBUMIN-SENSITIZED BROWN NORWAY RATS CAUSES IN VIRTO ACETYLCHOLINE AIRWAY HYPERRESPONSIVENESS
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Han, Jong-Hyun/한종현
J Otis/PM O´Byrne
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Abstract
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Airway hyperresponsiveness is a characteristic feature of asthma and has been associated with the airway inflammation present in asthamatic patients. We used ovalbumin sensitized Brown Norway rats to investigate the effects of sensitization on the contractile force of airway smooth muscle. Rats (150g, female) were actively sensitized by subcutaneous injection of 1.1ml Pertussis vaccine containing 10 μg alum-precipated ovalbumin. Rats were divided into two groups and exposed to and aerosol of either saline (n=6) or 5% ovalbumin (n=5), 24 hours after challenge, rats were euthanized and 8-10mm of the thoracic trachea was cut into equal segments and mounted ´in pairs´ in a tissue bath. The contractile response to acetylcholine (10^-7 -10^-4M) was measured by force displacement transducers under 0.5g loading tension. Allergen challenge increased the acetylcholine contractile responses. The mean contractile force (g/g dry weight) induced by acetylcholine 10^-5M in ovalbumin and saline challenged rats was 420±57 and 327±30 respectively (p〈0.05). The mean contractile force induced by acetylcholine 10^-4M in ovalbumin and saline challenged rats was 509±67 and 381±36 respectively (p〈0.05). Similar results were obtained with a second acetylcholine dose response curve performed 1h later. These indicate that the contractile force of airway smooth muscle is increased in sensitized rats after an ovalbumin challenge.
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